Rohini Kosoglu, Harris' domestic policy adviser, announced that she would be leaving the office in August. Meghan Groob, director of speechwriting, would also be leaving after only four months.
Kosoglu would serve on Harris' presidential campaign followed by a job on the transition team before moving to the vice president's office.
As a "brilliant and trusted leader who has been at my side for years from the U.S. Senate to the campaign trail to the White House," the vice president praised Kosoglu.
In a statement, Harris praised the woman for "bringing vision, strategic judgment, and a depth of experience as our administration has addressed some of the most urgent challenges facing our nation."
"She is an exceptional public servant and I am enormously grateful for her service. Rohini will remain a valued advisor and friend."
The high turnover rate in Harris' office during her first two and a half years in office has become a theme of her vice presidency, with a number of aides leaving at the end of 2021 and early of 2022
Former press secretary Symone Sanders and communications director Ashley Etienne both announced their departures from Harris' office at the end of last year.